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Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 121-122 doi: 10.15302/J-FASE-2017142

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Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 48-58 doi: 10.15302/J-FASE-2016114

Abstract: Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

Keywords： Puccinia striiformis f. sp. tritici     selfing     heterozygosity     virulence inheritance    

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Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

Frontiers of Structural and Civil Engineering 2019, Volume 13, Issue 6,   Pages 1338-1349 doi: 10.1007/s17009-019-0558-2

Abstract: main concentration being on energy absorption characteristics of concrete and the efficacy of fiber hybridization

Keywords： hybrid fiber-reinforced concrete     synergy     toughness     steel fibers     polypropylene fibers    

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Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 493-502 doi: 10.1007/s11684-022-0946-x

Abstract: Anaplastic lymphoma kinase (ALK) is the most common fusion gene involved in non-small cell lung cancer (NSCLC), and remarkable response has been achieved with the use of ALK tyrosine kinase inhibitors (ALK-TKIs). However, the clinical efficacy is highly variable. Pre-existing intratumoral heterogeneity (ITH) has been proven to contribute to the poor treatment response and the resistance to targeted therapies. In this work, we investigated whether the variant allele frequencies (VAFs) of ALK fusions can help assess ITH and predict targeted therapy efficacy. Through the application of next-generation sequencing (NGS), 7.2% (326/4548) of patients were detected to be ALK positive. On the basis of the adjusted VAF (adjVAF, VAF normalization for tumor purity) of four different threshold values (adjVAF < 50%, 40%, 30%, or 20%), the association of ALK subclonality with crizotinib efficacy was assessed. Nonetheless, no statistical association was observed between median progression-free survival (PFS) and ALK subclonality assessed by adjVAF, and a poor correlation of adjVAF with PFS was found among the 85 patients who received first-line crizotinib. Results suggest that the ALK VAF determined by hybrid capture-based NGS is probably unreliable for ITH assessment and targeted therapy efficacy prediction in NSCLC.

Keywords： ALK fusion     next-generation sequencing     fluorescence in situ hybridization     immunohistochemistry    

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Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

Quanlin DAI, Baifei HUANG, Zhongyi YANG, Jiangang YUAN, Junzhi YANG

Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 4,   Pages 449-458 doi: 10.1007/s11783-010-0250-x

Abstract: In this paper, the technique of suppression subtractive hybridization (SSH) was used to isolate and identify

Keywords： cadmium (Cd)     suppression subtractive hybridization    

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Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 3,   Pages 615-629 doi: 10.1007/s11705-020-1989-7

Abstract: electron microscopy assays, the metal-COD complexes revealed flower-like or granular structures after hybridizationHybridization in metal-based nanostructures could significantly enhance the initial conversion ratio

Keywords： cholesterol oxidase     metal-enzyme hybridization     nanostructures     sterol derivatives     bioconversion    

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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (CITRUS RETICULATA)

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 2,

Abstract:

Seedless fruits are desirable in the citrus fresh fruit market. Triploid production via diploid × tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus. Although ‘Orah’ mandarin has desirable organoleptic qualities, seeds in the fruits weaken its market competitiveness. To produce new seedless cultivars that are similar to ‘Orah’ mandarin, we performed three 2x × 4x crosses using ‘Orah’ mandarin as the seed parent to regenerate triploid plantlets. A total of 182 triploid and 36 tetraploid plantlets were obtained. By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, all of the triploids and tetraploids derived from these three crosses were proven to be hybrids. Also, we demonstrated that 2n megagametophyte formation in ‘Orah’ mandarin result in tetraploid production in these three interploidy crosses. These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers. Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%, indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels. These triploid hybrids enrich the germplasm available for seedless breeding. Moreover, the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.

 

Keywords： Citrus / 2n gamete / interploidy hybridization / pericentromeric SNP marker / second division restitution    

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Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system

Nanqi REN, Han KANG, Xiuheng WANG, Nan LI

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2,   Pages 277-282 doi: 10.1007/s11783-010-0226-x

Abstract: PAOs and GAOs were analyzed by fluorescent in situ hybridization (FISH), and intracellular polyphosphate

Keywords： fluorescent in situ hybridization (FISH)     tetrad forming organisms (TFOs)     temperature variation     enhanced    

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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (

Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO

Frontiers of Agricultural Science and Engineering   Pages 302-313 doi: 10.15302/J-FASE-2021385

Abstract: Seedless fruits are desirable in the citrus fresh fruit market. Triploid production via diploid × tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus. Although ‘Orah’ mandarin has desirable organoleptic qualities, seeds in the fruits weaken its market competitiveness. To produce new seedless cultivars that are similar to ‘Orah’ mandarin, we performed three 2x × 4x crosses using ‘Orah’ mandarin as the seed parent to regenerate triploid plantlets. A total of 182 triploid and 36 tetraploid plantlets were obtained. By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, all of the triploids and tetraploids derived from these three crosses were proven to be hybrids. Also, we demonstrated that 2n megagametophyte formation in ‘Orah’ mandarin result in tetraploid production in these three interploidy crosses. These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers. Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%, indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels. These triploid hybrids enrich the germplasm available for seedless breeding. Moreover, the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.

Keywords： Citrus     2n gamete     interploidy hybridization     pericentromeric SNP marker     second division restitution    

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Development of Perennial Wheat Through Hybridization Between Wheat and Wheatgrasses: A Review Review

Lei Cui,Yongkang Ren,Timothy D. Murray,Wenze Yan,Qing Guo,Yuqi Niu,Yu Sun,Hongjie Li

Engineering 2018, Volume 4, Issue 4,   Pages 507-513 doi: 10.1016/j.eng.2018.07.003

Abstract:

Wheatgrasses (Thinopyrum spp.), which are relatives of wheat (Triticum aestivum L.), have a perennial growth habit and offer resistance to a diversity of biotic and abiotic stresses, making them useful in wheat improvement. Many of these desirable traits from Thinopyrum spp. have been used to develop wheat cultivars by introgression breeding. The perennial growth habit of wheatgrasses inherits as a complex quantitative trait that is controlled by many unknown genes. Previous studies have indicated that Thinopyrum spp. are able to hybridize with wheat and produce viable/stable amphiploids or partial amphiploids. Meanwhile, efforts have been made to develop perennial wheat by domestication of Thinopyrum spp. The most promising perennial wheat–Thinopyrum lines can be used as grain and/or forage crops, which combine the desirable traits of both parents. The wheat–Thinopyrum lines can adapt to diverse agricultural systems. This paper summarizes the development of perennial wheat based on Thinopyrum, and the genetic aspects, breeding methods, and perspectives of wheat–Thinopyrum hybrids.

Keywords： Thinopyrum     Wheatgrass     Perennial     Triticum aestivum    

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Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 4,   Pages 616-624 doi: 10.1007/s11783-013-0505-4

Abstract: The fluorescence in situ hybridization (FISH) analysis revealed that the sludge was mainly dominated

Keywords： removal     anaerobic reaction time     nitrous oxide     polyhydroxyalkanoate     free nitrous acid     fluorescence in-situ hybridization    

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Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescence in situ Hybridization Article

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

Engineering 2022, Volume 9, Issue 2,   Pages 61-66 doi: 10.1016/j.eng.2021.05.012

Abstract: anaerobic sludge blanket reactor treating phenolic wastewater was investigated using fluorescence in situ hybridization

Keywords： Cryptanaerobacter     Fluorescence     in situ     hybridization     Anaerobic phenol degradation     Syntrophorhabdaceae    

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Title Author Date Type Operation

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Journal Article

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Journal Article

Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

Journal Article

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Journal Article

Identification of cadmium-induced genes in maize seedlings by suppression subtractive hybridization

Quanlin DAI, Baifei HUANG, Zhongyi YANG, Jiangang YUAN, Junzhi YANG

Journal Article

Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of steroids derivatives

Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang

Journal Article

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (CITRUS RETICULATA)

Journal Article

Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system

Nanqi REN, Han KANG, Xiuheng WANG, Nan LI

Journal Article

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (

Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO

Journal Article

Development of Perennial Wheat Through Hybridization Between Wheat and Wheatgrasses: A Review

Lei Cui,Yongkang Ren,Timothy D. Murray,Wenze Yan,Qing Guo,Yuqi Niu,Yu Sun,Hongjie Li

Journal Article

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

Journal Article

Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescence in situ Hybridization

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

Journal Article